K5BIO8 Gene expression and technologies personal learning checklist
AQA A-level Biology · Papers 2, 3
Name ____________________ Class __________ Date __________
Tick one box per statement. Red: need help. Amber: partly confident. Green: can do independently.
| I can | Red | Amber | Green |
|---|---|---|---|
| Sequence changes and protein consequences | |||
| predict effects of substitutions, additions, deletions and rearrangements on a specified gene | |||
| Stem cells, differentiation and potency | |||
| explain differentiation and distinguish totipotent, pluripotent, multipotent and unipotent potential | |||
| explain induced pluripotency and evaluate stem-cell applications | |||
| Transcription factors and epigenetic regulation | |||
| explain transcription-factor and oestrogen-receptor regulation | |||
| explain DNA methylation and histone acetylation as context-dependent regulatory mechanisms | |||
| interpret expression data while distinguishing RNA, protein abundance and functional outcomes | |||
| RNA interference and cancer regulation | |||
| explain sequence-specific RNA interference and evaluate knockdown evidence | |||
| compare benign and malignant growth and explain oncogene and tumour-suppressor roles | |||
| evaluate epigenetic and hormonal evidence in cancer and proposed targeted interventions | |||
| Genome projects and predicting proteins | |||
| explain applications and limits of using genome sequences to predict proteins and candidate antigens | |||
| Producing recombinant DNA and transformed cells | |||
| compare cDNA, restriction-based and synthetic routes to obtaining a desired sequence | |||
| explain construction of a suitable vector with an insert and regulatory regions | |||
| explain transformation, selection, screening and in vivo amplification | |||
| PCR amplification and electrophoresis | |||
| explain PCR requirements, stages and ideal amplification with realistic limits | |||
| interpret DNA electrophoresis using charge, size standards and detection limitations | |||
| Genetic screening and personalised medicine | |||
| explain labelled-probe detection and distinguish allele identification from outcome prediction | |||
| evaluate genetic counselling, personalised approaches and screening programmes | |||
| DNA profiling, applications and ethical evaluation | |||
| explain repeat variation and the biological principles of a multilocus DNA profile | |||
| interpret inheritance and forensic comparisons with appropriate evidential limits | |||
| evaluate gene therapy and recombinant applications using specific mechanisms and social consequences | |||
| Synoptic reasoning and written assessment | |||
| plan and write a relevant synoptic essay and evaluate biological evidence | |||
Next revision priority: __________________________________________________